Pollinators (GEP/GLP)
Apis mellifera
Acute oral and contact test
OECD 213/214. EPPO 170 (4), EPA 850.3020
Endpoint: mortality for short time exposure, up to 48 hrs.
Limit test (One single dose), Dose response test (5 dose levels to determine LR50).
Chronic feeding tests
OECD 245.
Endpoint: effects on bees after 10 days exposure period.
LC50, LDD50, NOEC and NOEDD are calculated.
Residual toxicity
OPPTS 850.3030.
Endpoint: Effects from different aged residue on foliage.
Larval toxicity test
OECD 237.
Endpoint: Mortality of larvae after a 72 hours application.
LD50 is calculated.
Bee brood study
OECD 75, Oomen et al.1992.
Endpoint: Impact on brood development (eggs and larvae).
Open field, greenhouse or tunnel test
EPPO 170(4), OECD 75.
Endpoint: mortality and behavior at long-term effects. (Brood status, colony health, foraging behavior)
Semi field brood test
OECD 75.
Mortality, flying activity and effects on the colonies (brood status)
Bombus spp.
Acute oral and contact test
OECD 246/247.
Endpoint: mortality for short time exposure, up to 48 hrs.
Limit test (One single dose), Dose response test (5 dose levels to determine LD50)
Residual toxicity
OPPTS 850.3030.
Enpoint: Mortality at different aged residue intervals on foliage.
Greenhouse or tunnel test
EPPO 170(4) adapted.
Endpoint: mortality and behavior at long-term period effects (brood status, colony health, foraging behavior)
Field test
EPPO 170.
Mortality, flying activity and effects on the colonies (brood status)